This file will allow you to calculate the equivalent cfu of your sample based on the absorbance obtained at 429 nm. First you will have to adjust the spectrophotometer to zero as you can read in the Legipid ® protocol and then insert the cuvette with the negative control. Repeat the zero and then enter the cuvettes with the different samples and record the obtained value. This is the Relative Absorbance (Ar).
Just enter that relative Absorbance data in the corresponding place in the table and press Enter. The cfu corresponding to that sample will automatically appear. That is, the equivalent cfu's which are in the 9 ml of the eluate (the analyzed volume in the cuvette).